Human Claudin-15 Antibody

Catalog # Availability Size / Price Qty
MAB10344-100
MAB10344-SP
Human Claudin-15 Antibody in Flow Cytometry
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Human Claudin-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin-15 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 540315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-15
Accession # P56746
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin-15  in HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP by Flow  Cytometry. View Larger

Detection of Claudin-15 in HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Claudin-15 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Claudin-15 Monoclonal Antibody (Catalog # MAB10344) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-15

CLDN15 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.

 

Entrez Gene IDs
24146 (Human); 60363 (Mouse); 304388 (Rat)
Alternate Names
claudin 15; Claudin15; Claudin-15; CLDN15; FLJ42715; MGC19536

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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