Human CMG-2/ANTXR2 Antibody

Catalog # Availability Size / Price Qty
AF2940
AF2940-SP
Human CMG-2/ANTXR2 Antibody in Flow Cytometry
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews

Human CMG-2/ANTXR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CMG‑2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse CMG-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CMG‑2/ANTXR2 isoform 1
Gln34-Asn317
Accession # P58335
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CMG‑2/ANTXR2 (Catalog # 2940-CM)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CMG‑2/ANTXR2 Monoclonal Antibody (Catalog # MAB29401).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CMG‑2/ANTXR2 in Human Monocytes by Flow Cytometry. View Larger

Detection of CMG‑2/ANTXR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human CMG‑2/ANTXR2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2940, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1‑5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

References
  1. Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  4. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  5. Santelli, E. et al. (2004) Nature 430:905.
  6. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  7. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  8. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  9. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  10. Wei, W. et al. (2006) Cell 124:1141.
  11. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Long Name
Capillary Morphogenesis Protein 2
Entrez Gene IDs
118429 (Human); 71914 (Mouse)
Alternate Names
anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2; CMG-2; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS

Product Datasheets

Citations for Human CMG-2/ANTXR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Differential dependence on N-glycosylation of anthrax toxin receptors CMG2 and TEM8.
    Authors: Friebe S, Deuquet J, van der Goot F
    PLoS ONE, 2015;10(3):e0119864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  2. Capillary morphogenesis gene 2 regulates adhesion and invasiveness of prostate cancer cells.
    Authors: Ye, Lin, Sanders, Andrew J, Sun, Ping-Hui, Mason, Malcolm, Jiang, Wen G
    Oncol Lett, 2014;7(6):2149-2153.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Therapeutic potential of capillary morphogenesis gene 2 extracellular vWA domain in tumourrelated angiogenesis.
    Authors: Ye L, Sun P, Sanders A, Martin T, Lane J, Mason M, Jiang W
    Int J Oncol, 2014;45(4):1565-73.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  4. Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function.
    Authors: Langer, Marybeth, Duggan, Elizabet, Booth, John Lel, Patel, Vineet I, Zander, Ryan A, Silasi-Mansat, Robert, Ramani, Vijay, Veres, Tibor Zo, Prenzler, Frauke, Sewald, Katherin, Williams, Daniel M, Coggeshall, Kenneth, Awasthi, Shanjana, Lupu, Florea, Burian, Dennis, Ballard, Jimmy Da, Braun, Armin, Metcalf, Jordan P
    Infect Immun, 2012;80(12):4374-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CMG-2/ANTXR2 Antibody

There are currently no reviews for this product. Be the first to review Human CMG-2/ANTXR2 Antibody and earn rewards!

Have you used Human CMG-2/ANTXR2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review