Detects human CNTF R alpha in direct ELISAs and Western blots. In Western blots, greater than 50% cross-reactivity with recombinant rat CNTF sR alpha is observed and less than 5% cross-reactivity with recombinant human (rh) IL-2 sR gamma and rhIL-6 sR is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha Gln23-Pro346 (predicted) Accession # P26992
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CNTF R alpha -enhanced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Human CNTF R alpha and 20 ng/mL Recombinant Human CNTF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutralization by Human CNTF R alpha Antibody. Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recombinant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recombinant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF R alpha
high-affinity CNTF receptor complex, which mediates the biological
action of CNTF, contains three proteins: the ligand-binding alpha subunit
(CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130.
Whereas LIF R beta and gp130 are widely expressed in many cell types, the
expression of CNTF R alpha is restricted to the central and peripheral
nervous systems. cDNAs encoding
human and rat CNTF R alpha share 94% amino acid (aa) sequence
identity. Human CNTF R alpha cDNA encodes a 372 aa precursor protein with a 22 aa residue signal peptide and four potential
glycoslyation sites. CNTF R alpha differs from other cytokine receptors in
that it lacks transmembrane and cytoplasmic domains and is anchored to
cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar
to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha ) can be
released from the cell surface by phosphatidylinositol-specific
phospholipase C. CNTF sR alpha can be released from skeletal muscle in
response to peripheral nerve injury and high concentrations of CNTF sR alpha
have also been detected in human cerebrospinal fluid. CNTF sR alpha binds
CNTF in solution and the complex can act on cells that express only LIF
R beta and gp130 but not CNTF R alpha.
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but also provides information about sample types, species, and experimental conditions.
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