Human CNTF R alpha Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CNTF R alpha  in direct ELISAs and Western blots. In Western blots, greater than 50% cross-reactivity with recombinant rat CNTF sR alpha is observed and less than 5% cross-reactivity with recombinant human (rh) IL-2 sR gamma and rhIL-6 sR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
    Gln23-Pro346 (predicted)
    Accession # P26992
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CNTF R alpha (Catalog # 303-CR)
  • Neutralization
    Measured by its ability to neutralize CNTF R alpha -enhanced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Human CNTF R alpha and 20 ng/mL Recombinant Human CNTF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral­ization by Human CNTF R alpha Antibody. Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recom­binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom­binant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF R alpha
The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.
  • Long Name:
    Ciliary Neurotrophic Factor Receptor alpha
  • Entrez Gene IDs:
    1271 (Human); 313173 (Rat)
  • Alternate Names:
    CNTF R alpha; CNTFR; CNTFRa
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010;17(0):4175.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Survival and axonal regeneration of off-center retinal ganglion cells of adult cats are promoted with an anti-glaucoma drug, nipradilol, but not BDNF and CNTF.
    Authors: Yata T, Nakamura M, Sagawa H, Tokita Y, Terasaki H, Watanabe M
    Neuroscience, 2007;148(1):53-64.
    Species: Feline
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
    Authors: Koh SW
    Invest. Ophthalmol. Vis. Sci., 2002;43(9):2887-96.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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