Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, COMET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1106301
View all Collagen IV alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide
Accession # P02462

Specificity

Detects recombinant human COL4A1 protein in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human Collagen IV  alpha 1 Antibody

COL4A1 in Human Kidney via seqIF™ staining on COMET™​

COL4A1 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.

COL4A1 in Human Liver via seqIF™ stainingon COMET™

COL4A1 was detected in immersion fixed paraffin-embedded sections of human Liver using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.

COL4A1 in Human Liver Carcinoma via seqIF™staining on COMET™

COL4A1 was detected in immersion fixed paraffin-embedded sections of human liver carcinoma using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.

Detection of Collagen IV  alpha 1 in Human Kidney.

Collagen IV  alpha 1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Collagen IV  alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen IV  alpha 1 in Human Liver.

Collagen IV  alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Collagen IV  alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen IV  alpha 1 in Human Liver Cancer.

Collagen IV  alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver cancer using Mouse Anti-Human Collagen IV  alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Collagen IV  alpha 1 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, human liver cancer and human liver

Multiplex Immunofluorescence

15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, liver and liver carcinoma

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen IV alpha 1

COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

Alternate Names

Arresten, COL4A1

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV alpha 1 Products

Product Documents for Human Collagen IV  alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Collagen IV  alpha 1 Antibody

For research use only

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Protocols

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