Human Collagen IV alpha 1 Antibody Summary
Accession # P02462
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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COL4A1 in Human Kidney via seqIF™ staining on COMET™ COL4A1 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
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COL4A1 in Human Liver via seqIF™ stainingon COMET™ COL4A1 was detected in immersion fixed paraffin-embedded sections of human Liver using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
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COL4A1 in Human Liver Carcinoma via seqIF™staining on COMET™ COL4A1 was detected in immersion fixed paraffin-embedded sections of human liver carcinoma using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
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Detection of Collagen IV alpha 1 in Human Kidney. Collagen IV alpha 1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Collagen IV alpha 1 in Human Liver. Collagen IV alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Collagen IV alpha 1 in Human Liver Cancer. Collagen IV alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver cancer using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB11758) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1
COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length. It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669. Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Product Datasheets
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