Human Collagen IV  alpha 1 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Collagen IV alpha 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 577238
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1
    Ser1441-Thr1669
    Accession # P02462
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Collagen IV  alpha 1 in T98G Human Cell Line. Collagen IV  alpha 1 was detected in immersion fixed T98G human glioblastoma cell line using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to perinuclear areas and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Collagen IV  alpha 1 in THP‑1 Human Cell Line. Collagen IV  alpha 1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human Collagen IV  alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1

COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

  • Entrez Gene IDs:
    1282 (Human); 12826 (Mouse); 290905 (Rat)
  • Alternate Names:
    alpha-1 chain; arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human Collagen IV  alpha 1 Antibody MAB6308
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Excellent
 IHC Human Anonymous 12/07/2016
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Immunohistochemistry Human Collagen IV  alpha 1 Antibody MAB6308
Immunohistochemistry: Human Collagen IV  alpha 1 Antibody [MAB6308]

Summary

ApplicationImmunohistochemistry
Sample TestedLung tissue
SpeciesHuman

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