Detects human Collagen IV alpha 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.
Monoclonal Mouse IgG1 Clone # 577238
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1 Ser1441-Thr1669 Accession # P02462
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Collagen IV alpha 1 in T98G Human Cell Line.
Collagen IV alpha 1 was detected in immersion fixed T98G human glioblastoma cell line using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to perinuclear areas and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Collagen IV alpha 1 in THP‑1 Human Cell Line.
Collagen IV alpha 1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1
COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length. It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669. Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Entrez Gene IDs:
1282 (Human); 12826 (Mouse); 290905 (Rat)
alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 126.96.36.199
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We have 1 review tested in 1 application: Immunohistochemistry.