Detection of Human Corin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Corin at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Corin in HK2 Human Cell Line.
Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.