Human Corin Antibody

  (1 citations)
(1 Review)
  
Product Details
Citations (1)
FAQs
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  • Species Reactivity
    Human
  • Specificity
    Detects human Corin in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 231443
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Corin
    Ser67-Asn1042
    Accession # Q9Y5Q5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Corin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Corin at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Corin in HK2 Human Cell Line. Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Corin
Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.
  • Entrez Gene IDs:
    10699 (Human)
  • Alternate Names:
    ATC2; atrial natriuteric peptide-converting enzyme; Corin; corin, serine peptidase; EC 3.4.21; EC 3.4.21.-; heart specific serine proteinase; Heart-specific serine proteinase ATC2; MGC119742; pro-ANP-convertase; Pro-ANP-Converting Enzyme; PRSC; serine protease; Transmembrane protease serine 10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells
    Sci Rep, 2016;6(0):32532.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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Read what people are saying who have used Human Corin Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Corin Antibody MAB2209 Array MAB2209
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Very Good
 ICC/IF Human Alexandra Blak 12/06/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human Corin Antibody MAB2209
Immunocytochemistry/Immunofluorescence: Human Corin Antibody [MAB2209].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested iPSC derived neural progenitor cells
Species Human

Other Experimental Details

Other Experimental Details Used the antibody for floorplate progenitor cells. It worked really great.

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