Human Corin Antibody

Catalog # Availability Size / Price Qty
AF2209
AF2209-SP
Human Corin Antibody in Western Blot
4 Images
Product Details
Citations (1)
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Human Corin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Corin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Corin
Ser67-Asn1042
Accession # Q9Y5Q5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Corin DuoSet ELISA Kit (Catalog # DY2209) for convenient development of a sandwich ELISA or the Human Corin Quantikine ELISA Kit (Catalog # DCRN00) for a complete optimized ELISA.

 
Immunohistochemistry
0.3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Corin by Western Blot. View Larger

Detection of Human Corin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # af2209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Corin at approximately 85 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Corin  in Human Liver. View Larger

Corin in Human Liver. Corin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Corin  in Human Heart. View Larger

Corin in Human Heart. Corin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Corin ELISA Standard Curve. Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Corin

Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.

Entrez Gene IDs
10699 (Human)
Alternate Names
ATC2; atrial natriuteric peptide-converting enzyme; Corin; corin, serine peptidase; EC 3.4.21; EC 3.4.21.-; heart specific serine proteinase; Heart-specific serine proteinase ATC2; MGC119742; pro-ANP-convertase; Pro-ANP-Converting Enzyme; PRSC; serine protease; Transmembrane protease serine 10

Product Datasheets

Citation for Human Corin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation.
    Authors: Kaushal G, Rognoni E, Lichtenberger B, Driskell R, Kretzschmar K, Hoste E, Watt F
    J Invest Dermatol, 2015;135(12):2926-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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