Human CRABP2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human CRABP2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CRABP2
    Met1-Glu138
    Accession # P29373
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CRABP2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, MDA‑MB‑468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRABP2
CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14 kDa cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138 amino acids (aa) in length and contains one NLS (aa 21‑31) and a series of beta -strands forming a beta -barrel that accommodates retinoic acid in its interior (aa 41‑135).
  • Long Name:
    Cellular Retinoic Acid Binding Protein 2
  • Entrez Gene IDs:
    1382 (Human); 12904 (Mouse); 29563 (Rat)
  • Alternate Names:
    cellular retinoic acid binding protein 2; Cellular retinoic acid-binding protein II; CRABP2; CRABP-II; CRABP-IIcellular retinoic acid-binding protein 2; RBP6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Activation of the CRABPII/RAR pathway by curcumin induces retinoic acid mediated apoptosis in retinoic acid resistant breast cancer cells
    Authors: P Thulasiram, G Garriga, V Danthuluri, DJ McAndrews, IQ Mohiuddin
    Oncol. Rep, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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