Detection of Human CRABP2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, MDA‑MB‑468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14 kDa cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138 amino acids (aa) in length and contains one NLS (aa 21‑31) and a series of beta -strands forming a beta -barrel that accommodates retinoic acid in its interior (aa 41‑135).
Cellular Retinoic Acid Binding Protein 2
Entrez Gene IDs:
1382 (Human); 12904 (Mouse); 29563 (Rat)
cellular retinoic acid binding protein 2; Cellular retinoic acid-binding protein II; CRABP-II; CRABP-IIcellular retinoic acid-binding protein 2; RBP6
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