Human Creatine Kinase MM/CKMM Antibody

Catalog # Availability Size / Price Qty
MAB5564
MAB5564-SP
Detection of Human Creatine Kinase MM/CKMM by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Creatine Kinase MM/CKMM Antibody Summary

Species Reactivity
Human
Specificity
Detects human Creatine Kinase, Muscle/CKMM in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 492731
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Creatine Kinase MM/CKMM antibody by Western Blot. View Larger

Detection of Human Creatine Kinase MM/CKMM by Western Blot. Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/ CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase MM/CKMM at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Creatine Kinase MM/CKMM antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/ CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Creatine Kinase MM/CKMM

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Long Name
Creatine Kinase, Muscle
Entrez Gene IDs
1158 (Human); 12715 (Mouse); 24265 (Rat)
Alternate Names
CKM; CKMM; CKMMcreatine kinase M-type; Creatine Kinase Isoenzyme; Creatine kinase M chain; Creatine Kinase MM; creatine kinase, muscle; creatine kinase-M; EC 2.7.3; EC 2.7.3.2; M-CK

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Creatine Kinase MM/CKMM Antibody

There are currently no reviews for this product. Be the first to review Human Creatine Kinase MM/CKMM Antibody and earn rewards!

Have you used Human Creatine Kinase MM/CKMM Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review