Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 492731
View all Creatine Kinase MM/CKMM Primary Antibodies »

Product Specifications

Immunogen

Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM

Specificity

Detects human Creatine Kinase, Muscle/CKMM in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Creatine Kinase MM/CKMM Antibody

Detection of Human Creatine Kinase MM/CKMM antibody by Western Blot.

Detection of Human Creatine Kinase MM/CKMM by Western Blot.

Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/ CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase MM/CKMM at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Creatine Kinase MM/CKMM antibody by Simple WesternTM.

Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/ CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Creatine Kinase MM/CKMM Antibody

Application
Recommended Usage

Simple Western

1 µg/mL
Sample: Human heart tissue

Western Blot

0.1 µg/mL
Sample: Human heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Creatine Kinase MM/CKMM

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Long Name

Creatine Kinase, Muscle

Alternate Names

CKM, CKMM, M-CK

Entrez Gene IDs

1158 (Human); 12715 (Mouse); 24265 (Rat)

Gene Symbol

CKM

Additional Creatine Kinase MM/CKMM Products

Product Documents for Human Creatine Kinase MM/CKMM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Creatine Kinase MM/CKMM Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human Creatine Kinase MM/CKMM Antibody

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