Human CRISP-3 Antibody Summary
Asn21-Tyr245 (Ser106Pro & Ala134Ser)
Accession # P54108.1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CRISP‑3 in Human Prostate. CRISP-3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CRISP-3 Monoclonal Antibody (Catalog # MAB23971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta -microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.
- Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
- Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
- Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
- Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
- Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
- Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
- Udby, L. et al. (2005) J. Androl. 26:333.
- Haendler, B. et al. (1993) Endocrinology 133:192.
- Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
- Bjartell, A. et al. (2006) Prostate 66:591.
- Liao, Q. et al. (2003) Histol. Histopathol. 18:245.
- Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
- Udby, L. et al. (2004) Biochemistry 43:12877.
- Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
- Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.
Citations for Human CRISP-3 Antibody
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Citations: Showing 1 - 2
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Eukaryotic expression, purification and structure/function analysis of native, recombinant CRISP3 from human and mouse.
Authors: Volpert, Marianna, Mangum, Jonathan, Jamsai, Duangpor, D'Sylva, Rebecca, O'Bryan, Moira K, McIntyre, Peter
Sci Rep, 2014;4(0):4217.
Sample Types: Cell Culture Supernates
Applications: Western Blot
Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
Authors: Matoso, Andres, Mukkada, Vincent, Lu, Shaolei, Monahan, Renee, Cleveland, Kelly, Noble, Lelia, Mangray, Shamlal, Resnick, Murray B
Mod Pathol, 2013;26(5):665-76.
Sample Types: Whole Tissue
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