Human CRISP-3 Antibody

Catalog # Availability Size / Price Qty
MAB23971
MAB23971-SP
CRISP‑3 in Human Prostate.
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Product Details
Citations (2)
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Human CRISP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRISP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross-reactivity with recombinant human CRISP-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 295203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRISP-3
Asn21-Tyr245 (Ser106Pro & Ala134Ser)
Accession # P54108.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CRISP‑3 in Human Prostate. View Larger

CRISP‑3 in Human Prostate. CRISP‑3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CRISP‑3 Monoclonal Antibody (Catalog # MAB23971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta -microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.

References
  1. Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
  2. Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
  3. Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
  4. Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
  5. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  6. Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
  7. Udby, L. et al. (2005) J. Androl. 26:333.
  8. Haendler, B. et al. (1993) Endocrinology 133:192.
  9. Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
  10. Bjartell, A. et al. (2006) Prostate 66:591.
  11. Liao, Q. et al. (2003) Histol. Histopathol. 18:245.
  12. Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
  13. Udby, L. et al. (2004) Biochemistry 43:12877.
  14. Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
  15. Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.
Long Name
Cysteine-rich Secretory Protein 3
Entrez Gene IDs
10321 (Human); 11572 (Mouse)
Alternate Names
Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa

Product Datasheets

Citations for Human CRISP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Eukaryotic expression, purification and structure/function analysis of native, recombinant CRISP3 from human and mouse.
    Authors: Volpert, Marianna, Mangum, Jonathan, Jamsai, Duangpor, D'Sylva, Rebecca, O'Bryan, Moira K, McIntyre, Peter
    Sci Rep, 2014;4(0):4217.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: WB
  2. Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
    Authors: Matoso, Andres, Mukkada, Vincent, Lu, Shaolei, Monahan, Renee, Cleveland, Kelly, Noble, Lelia, Mangray, Shamlal, Resnick, Murray B
    Mod Pathol, 2013;26(5):665-76.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Cell and Tissue Staining Kits

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Isotype Controls

Mouse IgG1 Isotype Control

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Secondary Antibodies

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