Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CTRP4/C1qTNF4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CTRP4/C1qTNF4
Leu17-Leu329
Accession # Q9BXJ3

Specificity

Detects human CTRP4/C1qTNF4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CTRP5 is observed and less than 1% cross-reactivity with rhCTRP1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CTRP4/C1qTNF4 Antibody

Detection of CTRP4/C1qTNF4 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of CTRP4/C1qTNF4 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Goat Anti-Human CTRP4/C1qTNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3456, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CTRP4/C1qTNF4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Western Blot

0.1 µg/mL
Sample: Recombinant Human CTRP4/C1qTNF4

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTRP4/C1qTNF4

Cq1TNF4, also known as CTRP4, is a secreted protein and is one of at least 9 paralogs of Acrp30/Adiponectin. Proteins in this family contain a globular C-terminal domain similar to that of the C1q and TNF ligand family. C1qTNF4 is the only family member that lacks the collagen-like domain but has two C1q domains. Mature human C1qTNF4 shares 92.4% amino acid sequence homology with the corresponding region of mouse C1qTNF4.

Long Name

C1q And Tumor Necrosis Factor Related Protein 4

Alternate Names

C1qTNF4, ZACRP4

Entrez Gene IDs

114900 (Human); 67445 (Mouse); 311184 (Rat)

Gene Symbol

C1QTNF4

UniProt

Q9BXJ3

Additional CTRP4/C1qTNF4 Products

Product Documents for Human CTRP4/C1qTNF4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CTRP4/C1qTNF4 Antibody

For research use only

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