Human CTRP5/C1qTNF5 Antibody Summary
Accession # AAH29485
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human CTRP5/C1qTNF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3167, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
C1qTNF5, also known as CTPR5, is a secreted protein that belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF-related protein family. All family members share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a
C‑terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse C1qTNF5 share 94% amino acid sequence identity.
Citation for Human CTRP5/C1qTNF5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
Biochem. J., 2008;416(2):161-77.
Sample Types: Recombinant Protein
Applications: Western Blot
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