Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.
Monoclonal Mouse IgG1 Clone # 60910
Protein A or G purified from ascites
E. coli-derived recombinant human CXCL6/GCP-2 Val40-Asn114 Accession # P80162
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human CXCL6/GCP‑2 Biotinylated Antibody (Catalog # BAF333)
Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)
Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL6/GCP‑2 and Neutralization by Human CXCL6/ GCP‑2 Antibody.
Recombinant Human CXCL6/ GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the
CXC chemokine family. It functions as
a neutrophil chemoattractant through
interactions with CXCR2.
Entrez Gene IDs:
chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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