Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.
Monoclonal Mouse IgG1 Clone # 60910
Protein A or G purified from ascites
E. coli-derived recombinant human CXCL6/GCP-2 Val40-Asn114 Accession # P80162
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human CXCL6/GCP‑2 Biotinylated Antibody (Catalog # BAF333)
Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)
Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL6/GCP‑2 and Neutralization by Human CXCL6/ GCP‑2 Antibody.
Recombinant Human CXCL6/ GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the
CXC chemokine family. It functions as
a neutrophil chemoattractant through
interactions with CXCR2.
Entrez Gene IDs:
chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.