Human CXCL6/GCP-2 Antibody MAB333: R&D Systems

Human CXCL6/GCP-2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 60910
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CXCL6/GCP-2
    Val40-Asn114
    Accession # P80162
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)
    • Human CXCL6/GCP-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CXCL6/GCP‑2 Antibody (Catalog # MAB333)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CXCL6/GCP‑2 Biotinylated Antibody (Catalog # BAF333)
  • ELISA Standard
     
    Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)
  • Neutralization
    Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL6/GCP‑2 and Neutral­ization by Human CXCL6/
GCP‑2 Antibody.

Recombinant Human CXCL6/
GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL6/GCP-2
CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the CXC chemokine family. It functions as a neutrophil chemoattractant through interactions with CXCR2.
  • Entrez Gene IDs:
    6372 (Human)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005;303(2):331-42.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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