Human CXCL6/GCP-2 Antibody

Catalog #: MAB333
2 Citations Datasheet / COA / SDS

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MAB333
MAB333-SP

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Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL6/GCP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.

Source

Monoclonal Mouse IgG₁ Clone # 60910

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CXCL6/GCP-2
Val40-Asn114
Accession # P80162

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)

Human CXCL6/GCP-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CXCL6/GCP‑2 Biotinylated Antibody (Catalog # BAF333)

Standard: Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)

Neutralization

Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody. Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP-2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP-2 Monoclonal Antibody (Catalog # MAB333). The ND₅₀ is typically 8-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL6/GCP-2

CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the CXC chemokine family. It functions as a neutrophil chemoattractant through interactions with CXCR2.

Entrez Gene IDs

6372 (Human)

Alternate Names

chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CXCL6/GCP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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