Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 328303
View all Cyclin D1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385

Specificity

Detects human Cyclin D1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cyclin D1 Antibody

Cyclin D1 antibody in COLO 205 Human Cell Line by Immunocytochemistry (ICC).

Cyclin D1 in COLO 205 Human Cell Line.

Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Cyclin D1 by Western Blot

Detection of Cyclin D1 by Western Blot

LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Cyclin D1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed COLO 205 human colorectal adenocarcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Reviewed Applications

Read 2 reviews rated 4 using MAB4314 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin D1

Cyclin D1 is a 44 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.

Alternate Names

Bcl-1, CCND1, Cyl-1, PRAD1

Entrez Gene IDs

595 (Human); 12443 (Mouse); 58919 (Rat)

Gene Symbol

CCND1

UniProt

P24385

Additional Cyclin D1 Products

Product Documents for Human Cyclin D1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cyclin D1 Antibody

For research use only

Citations for Human Cyclin D1 Antibody

Customer Reviews for Human Cyclin D1 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human Cyclin D1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate carcinoma
    Species: Human
    Verified Customer | Posted 11/19/2021
    Human Cyclin D1 Antibody MAB4314
  • Human Cyclin D1 Antibody
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: U266
    Species: Human
    Verified Customer | Posted 11/29/2016
    Immunocytochemistry/immunofluorescent staining of U266, MCF7, RPMI-8226 and Daudi with MAB4314.
    Cells immersion-fixed in 4% paraformaldehyde 10 minutes room temperature; permeabilized 0.5% Triton X-100 5 minutes; blocked 10% donkey serum/PBS 1 hour at room temperature; MAB4314 1/30 overnight at 4oC; Alexa 647 donkey F(ab')2 anti-mouse IgG 1/1000 1 hour at room termperature; images acquired on Zeiss AxioImager Cy5 filter 500ms exposure.
    Human Cyclin D1 Antibody MAB4314

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Protocols

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