Human Cystatin A Antibody Summary
Ile2-Phe98
Accession # P01040
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Cystatin A by Western Blot. Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1407) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Cystatin A by Flow Cytometry Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV+ populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV+ population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV+ cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fmicb.2024.1505900/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin A
Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).
- Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
- Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
- Sinha, A.A. et al. (2002) Cancer 94:3141.
Product Datasheets
Citation for Human Cystatin A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A Single Amino Acid Substitution in Porcine Reproductive and Respiratory Syndrome Virus Glycoprotein 2 Significantly Impairs Its Infectivity in Macrophages
Authors: J Chaudhari, RA Leme, K Durazo-Mar, S Sillman, AM Workman, HLX Vu
Viruses, 2022-12-18;14(12):.
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