Detection of Human Cytohesin‑1 by Western Blot.
Western blot shows lysates of NK‑92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Cytohesin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cytohesin‑1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cytohesin-1, previously called B2-1 or PSCD1 (pleckstrin homology, Sec7 and coiled-coil domain-containing protein 1), is a 398 amino acid (aa), 47 kDa member of the cytohesin family of guanine nucleotide exchange factors. The Sec7 domain (aa 62‑244) binds the integrin beta 2 subunit cytoplasmic domain, activating integrins via inside-out signaling, thus implicated in chemotaxis, adhesion, and cell spreading. The region of human Cytohesin-1 used as an immunogen shares 97% and 96% aa identity with mouse and rat Cytohesin-1, and 67% and 69% aa identity with human cytohesins 2 and 3, respectively. Although ubiquitously expressed, Cytohesin-1 is primarily found in immune system cells, and was cloned from a natural killer (NK) cell minus T helper cell line Jurkat library.
Entrez Gene IDs:
9267 (Human); 19157 (Mouse); 116691 (Rat)
B2-1; CYTH1; cytoadhesin 1; cytohesin 1; Cytohesin1; Cytohesin-1; D17S811ECYTOHESIN-1; FLJ34050; FLJ41900; homolog of secretory protein SEC7; PH, SEC7 and coiled-coil domain-containing protein 1; pleckstrin homology, Sec7 and coiled-coil domains 1; PSCD1; SEC7 homolog B2-1; SEC7
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