Human Cytohesin-1 Antibody

R&D Systems | Catalog # AF4807

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cytohesin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytohesin-1
Met1-Tyr102
Accession # Q15438

Specificity

Detects endogenous human Cytohesin-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cytohesin-1 Antibody

Detection of Human Cytohesin‑1 antibody by Western Blot.

Detection of Human Cytohesin‑1 by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Cytohesin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cytohesin-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cytohesin-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NK-92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytohesin-1

Cytohesin-1, previously called B2-1 or PSCD1 (pleckstrin homology, Sec7 and coiled-coil domain-containing protein 1), is a 398 amino acid (aa), 47 kDa member of the cytohesin family of guanine nucleotide exchange factors. The Sec7 domain (aa 62‑244) binds the integrin beta 2 subunit cytoplasmic domain, activating integrins via inside-out signaling, thus implicated in chemotaxis, adhesion, and cell spreading. The region of human Cytohesin-1 used as an immunogen shares 97% and 96% aa identity with mouse and rat Cytohesin-1, and 67% and 69% aa identity with human cytohesins 2 and 3, respectively. Although ubiquitously expressed, Cytohesin-1 is primarily found in immune system cells, and was cloned from a natural killer (NK) cell minus T helper cell line Jurkat library.

Alternate Names

B2-1, CYTH1, Cytohesin1, PSCD1, SEC7

Entrez Gene IDs

9267 (Human); 19157 (Mouse); 116691 (Rat)

Gene Symbol

CYTH1

UniProt

Q15438

Additional Cytohesin-1 Products

Product Documents for Human Cytohesin-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cytohesin-1 Antibody

For research use only

Citations for Human Cytohesin-1 Antibody

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