Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SULT1E1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
    Glu2-Leu295
    Accession # P50225
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 1A1/SULT1A1
Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity (2). The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters (3). Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers (2). The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility (4).
  • References:
    1. Falany, C. N. (1997) FASEB J. 11:206.
    2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
    3. Wilborn, T.W. et al. (1993) Mol. Pharm. 43:70.
    4. Gamage, N. U. et al. (2003) J. Biol. Chem. 278:7655.
    5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
    6. MacRae, I.J. et al. (2000) Biochemistry 39: 1613.
    7. Wu, Z.L. et al. (2002) Faseb J. 16:539.
  • Entrez Gene IDs:
    6817 (Human); 20887 (Mouse); 83783 (Rat)
  • Alternate Names:
    Aryl sulfotransferase 1; Cytosolic Sulfotransferase 1A1; EC 2.8.2; EC 2.8.2.1; HAST1/HAST2; Phenol sulfotransferase 1; Phenol-sulfating phenol sulfotransferase 1; P-PST 1; P-PST; PST; ST1A1; ST1A3; STP1 ; STP1MGC5163; STPMGC131921; sulfotransferase 1A1; sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1; SULT1A1; Thermostable phenol sulfotransferase; thermostable phenol sulfotransferase1; ts-PST; TSPST1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Contribution of nutritional deficit to the pathogenesis of the nonthyroidal illness syndrome in critical illness: a rabbit model study.
    Authors: Mebis L, Eerdekens A, Guiza F, Princen L, Derde S, Vanwijngaerden YM, Vanhorebeek I, Darras VM, Van den Berghe G, Langouche L
    Endocrinology, 2012;153(2):973-84.
    Species: Rabbit
    Sample Type: Tissue Homogenates
    Application: WB
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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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