Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 724429
View all Cytosolic Sulfotransferase 1B1/SULT1B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1B1/SULT1B1
Leu2-Ile296
Accession # O43704

Specificity

Detects human Cytosolic Sulfotransferase 1B1/SULT1B1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1C1, or rhSULT1E1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Cytosolic Sulfotransferase 1B1/SULT1B1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Cytosolic Sulfotransferase 1B1/SULT1B1 in Human Liver.

Cytosolic Sulfotransferase 1B1/SULT1B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Monoclonal Antibody (Catalog # MAB5959) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB5959 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1B1/SULT1B1

Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shares 72% amino acid sequence identity with mouse SULT1B1.

References

  1. Falany, C, N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
  4. Wang, J. et al. (1998) Mol. Pharmacol. 53:274.

Alternate Names

ST1B2, SULT1B1, SULT1B2

Entrez Gene IDs

27284 (Human); 56362 (Mouse); 64305 (Rat)

Gene Symbol

SULT1B1

UniProt

O43704

Additional Cytosolic Sulfotransferase 1B1/SULT1B1 Products

Product Documents for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

For research use only

Customer Reviews for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody (1)

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  • Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 02/20/2022
    Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody MAB5959

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Protocols

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