Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody Summary
Accession # O43704
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cytosolic Sulfotransferase 1B1/SULT1B1 in Human Liver. Cytosolic Sulfotransferase 1B1/SULT1B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Monoclonal Antibody (Catalog # MAB5959) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 1B1/SULT1B1
Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shares 72% amino acid sequence identity with mouse SULT1B1.
- Falany, C, N. (1997) FASEB J. 11:206.
- Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
- Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
- Wang, J. et al. (1998) Mol. Pharmacol. 53:274.
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