Human DcR3/TNFRSF6B Antibody

Catalog # Availability Size / Price Qty
MAB2135
MAB2135-SP
Human DcR3/TNFRSF6B Antibody in Data
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human DcR3/TNFRSF6B Antibody Summary

Species Reactivity
Human
Specificity
Detects human DcR3/TNFRSF6B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF R2, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 229812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DcR3/TNFRSF6B
Val24-His300
Accession # O95407
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize DcR3/TNFRSF6B-mediated inhibition of apoptosis in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 1 µg/mL Recombinant Human DcR3/TNFRSF6B Fc Chimera, 20 ng/mL Recombinant Human Fas Ligand/TNFSF6, and 10 µg/mL of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody. View Larger

DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DcR3/TNFRSF6B

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

References
  1. Pitti, R.M. et al. (1998) Nature 396:699.
  2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
  3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.
Long Name
Decoy Receptor 3
Entrez Gene IDs
8771 (Human); 107126607 (Cynomolgus Monkey)
Alternate Names
DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy

Product Datasheets

Citation for Human DcR3/TNFRSF6B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
    Authors: Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
    Leukemia, 2009;23(11):2139-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human DcR3/TNFRSF6B Antibody

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Human DcR3/TNFRSF6B Antibody
By Anonymous on 09/23/2016
Application: ELISA Sample Tested: Cell culture supernatant Species: Human