Human DEC-205/CD205 Antibody Summary
Accession # O60449
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human DEC‑205/CD205 by Western Blot. Western blot shows lysates of in vitro differentiated human immature myeloid dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEC-205/CD205 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human DEC‑205/CD205 by Western Blot. Western blot shows lysates of human thymus. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC‑205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for DEC‑205/CD205 at approximately 250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.
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