Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 523218
View all DEC-205/CD205 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449

Specificity

Detects human DEC‑205/CD205 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DEC‑205/CD205 Antibody

Detection of Human DEC‑205/CD205 antibody by Western Blot.

Detection of Human DEC‑205/CD205 by Western Blot.

Western blot shows lysates of in vitro differentiated human immature myeloid dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEC-205/CD205 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of Human DEC‑205/CD205 by Western Blot.

Western blot shows lysates of human thymus. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC‑205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for DEC‑205/CD205 at approximately 250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Applications for Human DEC‑205/CD205 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: In vitro differentiated human immature myeloid dendritic cells and human thymus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.

Alternate Names

CD205, DEC205, Ly75

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Gene Symbol

LY75

UniProt

O60449

Additional DEC-205/CD205 Products

Product Documents for Human DEC‑205/CD205 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DEC‑205/CD205 Antibody

For research use only

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail