Human DEPTOR/DEPDC6 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human DEPTOR/DEPDC6 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DEPTOR/DEPDC6
    Ala31-Gly158
    Accession # Q8TB45
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DEPTOR/DEPDC6 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7255) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DEPTOR/DEPDC6 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
DEPTOR/DEPDC6 in Human Kidney. DEPTOR/DEPDC6 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7255) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEPTOR/DEPDC6

DEPDC6 (DEP domain-containing protein 6; also DEPTOR) is a 47-48 kDa DEP (Disheveled/Egl10/Pleckstrin) domain-containing protein found in a wide variety of cells. It is a component of the IGF/Ins activated mTOR complexes 1 and 2 (TORC1 and 2) that promote S6K1 and Akt phosphorylation, respectively. Within TORC1, DEPDC6 acts as a natural inhibitor of mTOR kinase, suppressing S6K1 activity. Conversely, this inhibition of mTOR promotes TORC2 activation and Akt activity. In general, a decline in DEPDC6 activity promotes both an increase in cell size and lifespan. Human DEPDC6 is 409 amino acids (aa) in length. It is acetylated on Met1, contains two DEP domains (aa 36-119 and 145-219), and ends with a mTOR-interacting PDZ domain (aa 330-407). There are at least 13 utilized Ser/Thr phosphorylation sites. Although no splice variants have been reported, the highly orthologous mouse form of DEPDC6 does show a 34 aa substitution for the C-terminal 42 aa, suggesting that this splicing event may occur in human. Over aa 31-158, human DEPDC6 shares 98% aa sequence identity with mouse DEPDC6.

  • Long Name:
    DEP Domain Containing mTOR-interacting Protein
  • Entrez Gene IDs:
    64798 (Human); 97998 (Mouse)
  • Alternate Names:
    DEP domain containing 6; DEP domain containing mTOR interacting protein; DEP domain containing MTOR-interacting protein; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPDC6; DEPDC6FLJ12341; DEPTOR; DKFZp564B1778; FLJ12428; FLJ13854
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Basic

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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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