Human DISP1 Antibody

Catalog # Availability Size / Price Qty
MAB3549
MAB3549-SP

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DISP1 in BG01V Human Embryonic Stem Cells.
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Human DISP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DISP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DISP2 or rhSMAGP is observed.
Source
Monoclonal Mouse IgG2B Clone # 768213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DISP1
Glu212-Lys492 and Ser746-Thr984
Accession # Q96F81
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry DISP1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

DISP1 in BG01V Human Embryonic Stem Cells. DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human DISP1 Monoclonal Antibody (Catalog # MAB3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to membrane structures and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP1

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

Long Name
Dispatched 1
Entrez Gene IDs
84976 (Human); 68897 (Mouse); 289338 (Rat)
Alternate Names
DISP1; DISPA; DISPAFLJ43740; dispatched A; dispatched homolog 1 (Drosophila); Dispatched; DKFZp434I0428; MGC104180; MGC13130; MGC16796; protein dispatched homolog 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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