Human DISP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DISP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DISP2 or rhSMAGP is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 768213
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human DISP1
    Glu212-Lys492 and Ser746-Thr984
    Accession # Q96F81
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
DISP1 in BG01V Human Embryonic Stem Cells. DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human DISP1 Monoclonal Antibody (Catalog # MAB3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to membrane structures and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DISP1

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

  • Long Name:
    Dispatched 1
  • Entrez Gene IDs:
    84976 (Human); 68897 (Mouse); 289338 (Rat)
  • Alternate Names:
    DISP1; DISPA; DISPAFLJ43740; dispatched A; dispatched homolog 1 (Drosophila); Dispatched; DKFZp434I0428; MGC104180; MGC13130; MGC16796; protein dispatched homolog 1
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