Detection of Human DNA-PKcs by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DNA-dependent protein kinase catalytic subunit (DNA-PKCS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PKCS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.
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