Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DNA-PKCS
Thr3844-Trp4127
Accession # P78527

Specificity

Detects human DNA-PKCS in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DNA‑PKcs Antibody

Detection of Human DNA-PKcs antibody by Western Blot.

Detection of Human DNA-PKcs by Western Blot.

Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DNA‑PKcs Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNA-PKcs

DNA-dependent protein kinase catalytic subunit (DNA-PKCS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PKCS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Entrez Gene IDs

5591 (Human); 19090 (Mouse); 360748 (Rat)

Gene Symbol

PRKDC

UniProt

P78527

Additional DNA-PKcs Products

Product Documents for Human DNA‑PKcs Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DNA‑PKcs Antibody

For research use only

Related Research Areas

DNA Repair

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