Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DNMT3A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DNMT3A
Ala353-Lys486
Accession # Q9Y6K1

Specificity

Detects human DNMT3A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DNMT3A Antibody

DNMT3A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

DNMT3A in HeLa Human Cell Line.

DNMT3A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human DNMT3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6315) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

DNMT3A in HeLa Human Cell Line.

DNMT3A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Goat Anti-Human DNMT3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6315) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of DNMT3A by Western Blot

Detection of DNMT3A by Western Blot

CD133 interacts with DNMT1 depending on its C‐terminal cytoplasmic domain. D) The interaction between CD133 & the members of DNMT is examined by Co‐IP assay. Lysates of CD133+ are subjected to IP using anti‐CD133 antibody (Clone W6B3C1), followed by IB with anti‐CD133 (Clone W6B3C1), anti‐DNMT1, anti‐DNMT2, or anti‐DNMT3A antibodies. Whole‐cell lysates are analyzed by IB with anti‐CD133, anti‐DNMT1, anti‐DNMT2, or anti‐DNMT3A antibodies as input. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35798319), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of DNMT3A by Western Blot

Detection of DNMT3A by Western Blot

CD133 interacts with DNMT1 depending on its C‐terminal cytoplasmic domain. D) The interaction between CD133 & the members of DNMT is examined by Co‐IP assay. Lysates of CD133+ are subjected to IP using anti‐CD133 antibody (Clone W6B3C1), followed by IB with anti‐CD133 (Clone W6B3C1), anti‐DNMT1, anti‐DNMT2, or anti‐DNMT3A antibodies. Whole‐cell lysates are analyzed by IB with anti‐CD133, anti‐DNMT1, anti‐DNMT2, or anti‐DNMT3A antibodies as input. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35798319), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human DNMT3A Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed OVCAR‑3 human ovarian carcinoma cell line and immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNMT3A

DNMT3A (DNA [cytosine-5]-methyltransferase 3A) is a 130 kDa member of the C5-methlytransferase family of molecules. It is expressed both during development and in virtually all adult tissues except for small intestine. DNMT3A is a nuclear protein that is found associated with heterochromatin. It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer. It also interacts with SETDB1 to form a transcriptional repressor. Human DNMT3A is 912 amino acids (aa) in length. It contains one ProTrpTrpPro domain (aa 292-350) and one ADD-type zinc finger region (aa 494-586). There is one 100 kDa splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin. Other potential splice variants show a 16 aa substitution for aa 151-912, and a 24 aa substitution for aa 1-213. Over aa 353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A. 

Long Name

DNA [Cytosine-5-]-Methyltransferase 3A

Alternate Names

DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA Methyltransferase 3 Alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA, TBRS

Entrez Gene IDs

1788 (Human); 13435 (Mouse); 444984 (Rat)

Gene Symbol

DNMT3A

UniProt

Q9Y6K1 (Human)

Additional DNMT3A Products

Product Documents for Human DNMT3A Antibody

Certificate of Analysis

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Product Specific Notices for Human DNMT3A Antibody

For research use only

Related Research Areas

Adult Neurogenesis
Epigenetics

Citations for Human DNMT3A Antibody

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