Human Drebrin 1 Antibody

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  • Species Reactivity
    Human
  • Specificity
    Detects human Drebrin 1 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 838102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Drebrin 1
    Asn482-Asp649 (Ser553Pro)
    Accession # Q16643
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Drebrin 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Drebrin 1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Drebrin 1 in HeLa Human Cell Line. Drebrin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Drebrin 1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Drebrin 1 at approximately 162 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Drebrin 1
Drebrin 1 (DBN-1 [developmentally-regulated brain protein1]; also drebrin-E/E2 [Embryonic]) is an intracellular member of the ADF-H (actin-depolymerizing factor-H) family of actin binding proteins. Although its predicted MW is 72 kDa, it runs anomalously at 115-116 kDa in SDS-PAGE. It is expressed by neurons, gastric Parietal cells, astrocytes, distal convoluted tubule epithelium and proton-secreting intercalated cells of the renal collecting duct. Drebrin 1 interacts with multiple partners near the membrane. It links connexin-43 and F-actin, thereby stabilizing membrane gap junctions. It also binds to EB3 (end-binding protein 3) on microtubules, facilitating actin-microtubule interactions. Human Drebrin 1 is 649 amino acids (aa) in length. It contains one actin depolymerizing homology domain (aa 3-134), an actin-binding region (≈ aa 150-300), and two HOMER binding motifs (aa 539-543 and 617-621). There are at least 10 utilized Ser/Thr phosphorylation sites and one utilized Tyr phosphorylation site. Alternative splicing generates drebrin-A (Adult), a 124-126 kDa isoform that contains a 46 aa insert after Gly319. Drebrin-A is found in neurons and possibly podocytes, and is associated with dendritic spines where it inhibits the interaction of F-actin with alpha -actinin and tropomyosin. This favors the generation of excitatory impulses in neurons. Three other potential isoform variants are noted. One utilizes an alternative start site at Met64, a second shows a 60 aa substitution for aa 1-110, and a third contains a 28 aa substitution for aa 4-29. Over aa 482-649, human Drebrin 1 shares 84% aa sequence identity with mouse Drebrin 1.
  • Entrez Gene IDs:
    1627 (Human); 56320 (Mouse); 81653 (Rat)
  • Alternate Names:
    D0S117E; DBN1; Developmentally-regulated brain protein; DKFZp434D064; Drba; Drebrin 1; Drebrin A; Drebrin E; Drebrin E2; drebrin; drebrin-1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Drebrin 1 Antibody MAB7739 Array MAB7739
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Very Good
 ICC/IF Mouse Anonymous 04/21/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human Drebrin 1 Antibody MAB7739
Immunocytochemistry/Immunofluorescence: Human Drebrin 1 Antibody [MAB7739].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Kidney tissue
Species Mouse

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