Human DSCAM Long Isoform Antibody

Catalog # Availability Size / Price Qty
AF3666
AF3666-SP
Human DSCAM Long Isoform Antibody in Western Blot
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human DSCAM Long Isoform Antibody Summary

Species Reactivity
Human
Specificity
Detects human DSCAM in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DSCAM-L1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSCAM long isoform
Glu18-Met1595
Accession # O60469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Human DSCAM by Western Blot. View Larger

Detection of Recombinant Human DSCAM by Western Blot. Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM‑L1 (Catalog # 3315-DL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry DSCAM in A172 Human Cell Line. View Larger

DSCAM in A172 Human Cell Line. DSCAM was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSCAM

Human DSCAM is a member of the Ig superfamily that maps to a region in chromosome 21 associated with Down Syndrome mental retardation. It encodes a 230 kDa type I membrane protein with a large extracellular domain comprising ten C2 type Ig like domains and six fibronectin type III repeats. The intracellular domain of human DSCAM does not share significant sequence homology with Drosophila DSCAM. Human DSCAM is expressed in axonal and dendritic processes of the central and peripheral nervous system. It is involved in hemophilia interactions and has been implicated in neurogenesis. By alternative splicing, a soluble form of human DSCAM that is identical to the transmembrane form through residue 1571 has been described. The extracellular domain of human and mouse DSCAM share 98% amino acid sequence homology.

Long Name
Down Syndrome Cell Adhesion Molecule
Entrez Gene IDs
1826 (Human); 13508 (Mouse)
Alternate Names
CHD2-42; CHD2-52; Down syndrome cell adhesion molecule; DSCAM; human CHD2-52 down syndrome cell adhesion molecule, 10CHD2

Product Datasheets

Citations for Human DSCAM Long Isoform Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. DSCAM promotes axon fasciculation and growth in the developing optic pathway
    Authors: FM Bruce, S Brown, JN Smith, PG Fuerst, L Erskine
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Neurite arborization and mosaic spacing in the mouse retina require DSCAM.
    Authors: Fuerst PG, Koizumi A, Masland RH, Burgess RW
    Nature, 2008;451(7177):470-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

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Secondary Antibodies

Reviews for Human DSCAM Long Isoform Antibody

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Human DSCAM Long Isoform Antibody
By Anonymous on 01/13/2018
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human