Human DSCAM Long Isoform Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human DSCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human DSCAM-L1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DSCAM long isoform
    Glu18-Met1595
    Accession # O60469
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human DSCAM by Western Blot. Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM‑L1 (Catalog # 3315-DL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
DSCAM in A172 Human Cell Line. DSCAM was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DSCAM

Human DSCAM is a member of the Ig superfamily that maps to a region in chromosome 21 associated with Down Syndrome mental retardation. It encodes a 230 kDa type I membrane protein with a large extracellular domain comprising ten C2 type Ig like domains and six fibronectin type III repeats. The intracellular domain of human DSCAM does not share significant sequence homology with Drosophila DSCAM. Human DSCAM is expressed in axonal and dendritic processes of the central and peripheral nervous system. It is involved in hemophilia interactions and has been implicated in neurogenesis. By alternative splicing, a soluble form of human DSCAM that is identical to the transmembrane form through residue 1571 has been described. The extracellular domain of human and mouse DSCAM share 98% amino acid sequence homology.

  • Long Name:
    Down Syndrome Cell Adhesion Molecule
  • Entrez Gene IDs:
    1826 (Human); 13508 (Mouse)
  • Alternate Names:
    CHD2-42; CHD2-52; Down syndrome cell adhesion molecule; DSCAM; human CHD2-52 down syndrome cell adhesion molecule, 10CHD2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. DSCAM promotes axon fasciculation and growth in the developing optic pathway
    Authors: FM Bruce, S Brown, JN Smith, PG Fuerst, L Erskine
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Neurite arborization and mosaic spacing in the mouse retina require DSCAM.
    Authors: Fuerst PG, Koizumi A, Masland RH, Burgess RW
    Nature, 2008;451(7177):470-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
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