Human E6AP/UBE3A Antibody

Catalog #: MAB11498 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11498-100
MAB11498-SP

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Detection of Human E6AP/UBE3A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E6AP/UBE3A Antibody Summary

Species Reactivity

Human

Specificity

Detects human E6AP/UBE3A in Western blots.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 1077332

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A
Met1-Leu875
Accession # Q05086

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Jurkat human acute T cell leukemia cell line

Simple Western

20 µg/mL

K562 human chronix myelogenous leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human E6AP/UBE3A by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E6AP/UBE3A at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human E6AP/UBE3A by Simple Western^TM. Simple Western shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for E6AP/UBE3A at approximately 106 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

References

Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Long Name

Human Papillomavirus E6-associated Protein

Entrez Gene IDs

7337 (Human); 22215 (Mouse); 361585 (Rat)

Alternate Names

ANCR; ANCREPVE6AP; AS; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; E6AP; E6-AP; EC 6.3.2.-; EPVE6AP; FLJ26981; HPVE6A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin protein ligase E3A; ubiquitin-protein ligase E3A