Human EBI2 Antibody

Catalog # Availability Size / Price Qty
MAB10272-100
MAB10272-SP
Detection of EBI2  in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow  Cytometry.
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Human EBI2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EBI2 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 486633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived transfected withrecombinant human EBI2
Accession # P32249
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of EBI2  in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow  Cytometry. View Larger

Detection of EBI2 in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) EBI2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2A Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of EBI2  in A549 Human Cell Line by Flow  Cytometry. View Larger

Detection of EBI2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI2

Epstein-Barr virus-induced G-protein coupled receptor 2, also known as EBI2 or GPR183 is a G-protein coupled receptor (GPCR) expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, and monocytes/macrophages. The GPR183 gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of the Burkitt's lymphoma cell line BL41. GPR183 collaborates with CXCR5 to mediate B cell homing within a lymph node, probably by forming a heterodimer with CXCR5 that affects its interaction with CXCL13

Long Name
Epstein-Barr Virus Induced Gene 2
Entrez Gene IDs
1880 (Human); 321019 (Mouse); 679975 (Rat)
Alternate Names
EBI2; EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor); EBV-induced G-protein coupled receptor 2; Epstein-Barr virus-induced G-protein coupled receptor 2; G protein-coupled receptor 183; GPR183; G-protein coupled receptor 183

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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