Detection of Human eIF2 alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human eIF2 alpha by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2 alpha at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF2 alpha
elF2 alpha (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the elF2 protein, an important regulator of translation initiation. Phosphorylation of elF2 alpha on Ser 52 increases the affinity of elF2 alpha for elF2B, a guanine nucleotide exchange factor needed for the recycling of elF2-GDP to elF2-GTP. Reduction of elF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all elF2 alpha kinases.
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