Human eIF2 alpha Antibody

Catalog # Availability Size / Price Qty
AF3997
AF3997-SP
Detection of Human eIF2 alpha  by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human eIF2 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse, and rat eIF2 alpha in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human eIF2 alpha
Met1-Asp315
Accession # Q53XC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human eIF2a antibody by Western Blot. View Larger

Detection of Human eIF2 alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human eIF2a antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human eIF2 alpha by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2a at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF2 alpha

elF2 alpha (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the elF2 protein, an important regulator of translation initiation. Phosphorylation of elF2 alpha on Ser 52 increases the affinity of elF2 alpha for elF2B, a guanine nucleotide exchange factor needed for the recycling of elF2-GDP to elF2-GTP. Reduction of elF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all elF2 alpha kinases.

Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Entrez Gene IDs
1965 (Human); 13665 (Mouse); 54318 (Rat)
Alternate Names
eIF2 alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1

Product Datasheets

Citations for Human eIF2 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Silvestrol Inhibits Chikungunya Virus Replication
    Authors: L Henss, T Scholz, A Grünweller, BS Schnierle
    Viruses, 2018;10(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection
    Authors: PM Tiwari, D Vanover, KE Lindsay, SS Bawage, JL Kirschman, S Bhosle, AW Lifland, C Zurla, PJ Santangelo
    Nat Commun, 2018;9(1):3999.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human eIF2 alpha Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human eIF2 alpha Antibody
By Jothi Jayavel on 01/11/2018
Application: Western Blot Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Human eIF2 alpha Antibody
By Dharmaraja Allimuthu on 12/27/2017
Application: Western Blot Sample Tested: Embryonic stem cells Species: Human