Human Endothelin-3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Endothelin‑3.
  • Source
    Monoclonal Mouse IgG1 Clone # 3B6
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human Endothelin-3 synthetic peptide
    Accession # P14138
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Endothelin‑3 in LNCaP Human Cell Line. Endothelin‑3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin‑3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endothelin-3
Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield the secreted and inactive big endothelins. Big
Endothelin-3 is further cleaved by Kell blood group antigen endopeptidases to generate the 21 amino acid (aa) bioactive mature peptide. It interacts primarily with the 7TM receptor EDNRB to induce a variety of effects including bronchoconstriction, arteriolar vasoconstriction, gall bladder contraction, insulin and norepinephrine release, and the maintenance and development of melanocytes and neural crest progenitors. Mature human Endothelin-3 shares 100% aa sequence identity with mouse and rat Endothelin-3.
  • Entrez Gene IDs:
    1908 (Human); 13616 (Mouse); 366270 (Rat)
  • Alternate Names:
    EDN3; endothelin 3; Endothelin3; Endothelin-3; ET3; ET-3; HSCR4; MGC15067; MGC61498; PPET3; preproendothelin-3; truncated endothelin 3; WS4B
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