Human Endothelin-3 Antibody
Human Endothelin-3 Antibody Summary
Accession # P14138
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Endothelin‑3 in LNCaP Human Cell Line. Endothelin-3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin-3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield the secreted and inactive big endothelins. Big
Endothelin-3 is further cleaved by Kell blood group antigen endopeptidases to generate the 21 amino acid (aa) bioactive mature peptide. It interacts primarily with the 7TM receptor EDNRB to induce a variety of effects including bronchoconstriction, arteriolar vasoconstriction, gall bladder contraction, insulin and norepinephrine release, and the maintenance and development of melanocytes and neural crest progenitors. Mature human Endothelin-3 shares 100% aa sequence identity with mouse and rat Endothelin-3.
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