Detection of Human EPCR by Western Blot. Western blot shows lysates of human skin tissue, human placenta tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EPCR in Human Liver. EPCR was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. Protein C is a vitamin K-dependent serine protease that plays a major
role in blood coagulation. Binding of Protein C to EPCR leads to the
proteolytic activation of PAR1 (protease-activated receptor 1) on
endothelial cells and subsequent up-regulation of Protein C-induced
extracellular domain of human and mouse EPCR shares approximately 61%
amino acid sequence homology.
Endothelial Protein C Receptor
Entrez Gene IDs:
10544 (Human); 19124 (Mouse)
APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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