Detection of Human Eps8 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Eps8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5529) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Eps8 at approximately 97 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Eps8 (EGF R pathway substrate 8) is a 97 kDa member of the Eps family of proteins. It is ubiquitously expressed and serves as a signaling molecule for multiple RTKs, including FGF R, EGF R and PDGF R. Upon activation, Eps8 promotes endocytosis through Rab5, and Rac activation via IRSp53, Abi-1 and Sos-1. Human Eps8 is 822 amino acids (aa) in length. It contains a PH-like domain (aa 62‑173), an EGF R binding region (aa 296‑362), an SH3 domain that mediates homodimerization (aa 535‑587) and a Sos-1 interaction site (aa 648‑821). There is one potential alternate start site at Met261, and a splice variant that shows a 15 aa substitution for aa 676‑822.
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