Human ER beta /NR3A2 Antibody

Catalog # Availability Size / Price Qty
MAB71061-100
MAB71061-SP
Human ER beta /NR3A2 Antibody in Immunocytochemistry
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Human ER beta /NR3A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ER beta /NR3A2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1013506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ER beta /NR3A2
Met1-Gly318
Accession # Q92731
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ER beta/NR3A2 in MCF‑7 human breast cancer cell line. View Larger

ER beta/NR3A2 in MCF‑7 human breast cancer cell line. ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line (left panel; positive staining) and THP-1 human acute monocytic leukemia cell line (right panel; negative staining) using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB71061) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER beta/NR3A2

Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name
Estrogen Receptor beta
Entrez Gene IDs
2100 (Human)
Alternate Names
ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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