Human ER beta/NR3A2 Antibody (1013506)

R&D Systems | Catalog # MAB71061

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1013506
View all ER beta/NR3A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ER beta /NR3A2
Met1-Gly318
Accession # Q92731

Specificity

Detects human ER beta /NR3A2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ER beta/NR3A2 Antibody (1013506)

ER beta/NR3A2 antibody in MCF-7 human breast cancer cell line by Immunocytochemistry (ICC).

ER beta/NR3A2 in MCF‑7 human breast cancer cell line.

ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line (left panel; positive staining) and THP-1 human acute monocytic leukemia cell line (right panel; negative staining) using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB71061) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human ER beta/NR3A2 Antibody (1013506)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ER beta/NR3A2

Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name

Estrogen Receptor beta

Alternate Names

ESR2, NR3A2

Entrez Gene IDs

2100 (Human)

Gene Symbol

ESR2

UniProt

Q92731

Additional ER beta/NR3A2 Products

Product Documents for Human ER beta/NR3A2 Antibody (1013506)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ER beta/NR3A2 Antibody (1013506)

For research use only

Citations for Human ER beta/NR3A2 Antibody (1013506)

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail