Human Erythropoietin R Antibody Summary
Accession # P19235
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Erythropoietin R by Simple WesternTM. Simple Western lane view shows lysates of UT-7 human acute myeloid leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Erythropoietin R at approximately 76 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The genes for human and mouse Epo R have been cloned and characterized. The full-length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
- Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
- Youssoufian, H. et al. (1993) Blood 9:2223.
- Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
- Baynes, R.D. et al. (1993) Blood 82:2088.
Citations for Human Erythropoietin R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010;5(2):e9246.
Sample Types: Serum
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
J Neurosci Methods, 0;164(1):50-8.
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