Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 702321
View all EVI-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112

Specificity

Detects human EVI-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EVI‑1 Antibody

EVI-1 antibody in SK-OV-3 Human Cell Line by Immunocytochemistry (ICC).

EVI‑1 in SK‑OV‑3 Human Cell Line.

EVI-1 was detected in immersion fixed SK-OV-3 human ovarian adenocarcinoma cell line using Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB75061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human EVI‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SK‑OV‑3 human ovarian adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EVI-1

Ectopic virus integration site 1 (EVI-1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI-1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI-1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI-1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI-1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI-1 shares 94% aa sequence identity with mouse and rat EVI-1.

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Entrez Gene IDs

2122 (Human); 14013 (Mouse); 294924 (Rat)

Gene Symbol

MECOM

UniProt

Q03112

Additional EVI-1 Products

Product Documents for Human EVI‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EVI‑1 Antibody

For research use only

Related Research Areas

Hematopoietic Stem Cell Markers

Citations for Human EVI‑1 Antibody

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