Detection of Human FABP5/E‑FABP by Western Blot.
Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E‑FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/ E‑FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP5 in HUVEC Human Cells.
FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FABP5, also known as epidermal fatty acid binding protein (E-FABP), is expressed in skin, lens, adipose tissue, endothelial cells, heart, brain and placenta. FABP-5 is associated with keratinocytes and
adipocytes, and is suggested to promote fatty acid availability to enzymes,
protect cell structures from fatty acid attack, and target fatty acids to
nuclear transcription factors. Human
FABP-5 shares 80%, 81%, and 92% aa identity with mouse, rat and bovine FABP-5,
Fatty Acid-Binding Protein 5
Entrez Gene IDs:
2171 (Human); 16592 (Mouse); 140868 (Rat)
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog
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but also provides information about sample types, species, and experimental conditions.
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