Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 311215
View all FABP5/E-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469

Specificity

Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human FABP5/E‑FABP Antibody

Detection of Human FABP5/E-FABP antibody by Western Blot.

Detection of Human FABP5/E‑FABP by Western Blot.

Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP5 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

FABP5 in HUVEC Human Cells.

FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-con-jugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human FABP5/E-FABP Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human FABP5/E‑FABP Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FABP5/E‑FABP Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Knockout Validated

FABP5/E‑FABP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FABP5/E‑FABP knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: Human heart tissue, human brain (cerebellum) tissue, and human placenta tissue

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP5/E-FABP

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is expressed in skin, lens, adipose tissue, endothelial cells, heart, brain and placenta. FABP-5 is associated with keratinocytes and adipocytes, and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors.  Human FABP-5 shares 80%, 81%, and 92% aa identity with mouse, rat and bovine FABP-5, respectively.

Long Name

Fatty Acid-Binding Protein 5

Alternate Names

E-FABP, EFABP

Entrez Gene IDs

2171 (Human); 16592 (Mouse); 140868 (Rat)

Gene Symbol

FABP5

UniProt

Q01469

Additional FABP5/E-FABP Products

Product Documents for Human FABP5/E‑FABP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FABP5/E‑FABP Antibody

For research use only

Citations for Human FABP5/E‑FABP Antibody

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Protocols

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