Human FADD Antibody Summary
Accession # Q13158
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FADD by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human FADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FADD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fas-Associating protein with Death Domain (FADD), also known as MORT1, is a 25 kDa adaptor protein that mediates signaling of death domain containing TNF receptor superfamily members. FADD interacts with the death domain of FAS (CD95) and TNFR-1 and recruits procaspase-8 to the Fas signaling complex, initiating caspase activation and apoptosis.
Citation for Human FADD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells
Authors: WR English, H Ireland-Ze, AH Baker, TD Littlewood, MR Bennett, G Murphy
PLoS ONE, 2018;13(4):e0195116.
Sample Types: Cell Lysates
Applications: Western Blot
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