Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FADD Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FADD
Met1-Ser208
Accession # Q13158

Specificity

Detects endogenous human FADD in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FADD Antibody

Detection of Human FADD antibody by Western Blot.

Detection of Human FADD by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human FADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FADD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human FADD Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FADD

Fas-Associating protein with Death Domain (FADD), also known as MORT1, is a 25 kDa adaptor protein that mediates signaling of death domain containing TNF receptor superfamily members. FADD interacts with the death domain of FAS (CD95) and TNFR-1 and recruits procaspase-8 to the Fas signaling complex, initiating caspase activation and apoptosis.

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Entrez Gene IDs

8772 (Human)

Gene Symbol

FADD

UniProt

Q13158

Additional FADD Products

Product Documents for Human FADD Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FADD Antibody

For research use only

Citations for Human FADD Antibody

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