Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse FAM3C is observed and 5% cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, and rhFAM3D is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human FAM3C Gln25-Asp227 Accession # Q92520
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FAM3C by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FAM3C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAM3C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FAM3C, also known as predicted Osteoblast protein, is a secreted 22 kDa
glycoprotein belonging to the cytokine-like protein family. Members of
this family share a four helix bundle secondary structure similar to
that found in many cytokines. FAM3 is ubiquitously expressed in
multiple tissues. The amino acid sequence of mature human FAM3C is 94%
and 98% identical to that of mouse and canine FAM3C, respectively.
Family With Sequence Similarity 3, Member C
Entrez Gene IDs:
10447 (Human); 27999 (Mouse); 312159 (Rat)
D6WSU176e; FAM3C; family with sequence similarity 3, member C; GS3876; ILEI; ILEIInterleukin-like EMT inducer; predicted osteoblast protein
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