Human FAM3C Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse FAM3C is observed and 5% cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, and rhFAM3D is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FAM3C
    Gln25-Asp227
    Accession # Q92520
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FAM3C by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FAM3C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAM3C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAM3C
FAM3C, also known as predicted Osteoblast protein, is a secreted 22 kDa glycoprotein belonging to the cytokine-like protein family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3 is ubiquitously expressed in multiple tissues. The amino acid sequence of mature human FAM3C is 94% and 98% identical to that of mouse and canine FAM3C, respectively.
  • Long Name:
    Family With Sequence Similarity 3, Member C
  • Entrez Gene IDs:
    10447 (Human); 27999 (Mouse); 312159 (Rat)
  • Alternate Names:
    D6WSU176e; FAM3C; family with sequence similarity 3, member C; GS3876; ILEI; ILEIInterleukin-like EMT inducer; predicted osteoblast protein
Related Research Areas
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 138  
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 6
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 2  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 4  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 3  
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 4
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Donkey Anti-Goat IgG Antibody

WB AF109 5
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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