Human Fas/TNFRSF6/CD95 Antibody

R&D Systems | Catalog # AF326

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Fas/TNFRSF6/CD95 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fas

Specificity

Detects human Fas in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat Fas and recombinant mouse Fas is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Fas/TNFRSF6/CD95 Antibody

Detection of Human Fas/TNFRSF6/CD95 antibody by Western Blot.

Detection of Human Fas/TNFRSF6/CD95 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fas/TNFRSF6/CD95 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Fas / TNFRSF6 / CD95 ELISA Standard Curve

Human Fas / TNFRSF6 / CD95 ELISA Standard Curve

Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Fas/TNFRSF6/CD95 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF326 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the new TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated
T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas. Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL‑mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

References

  1. Nagata, S. and P. Golstein (1995) Science 267:1449.
  2. Nagata, S. (1997) Cell 88:355.
  3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
  4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Gene Symbol

FAS

Additional Fas/TNFRSF6/CD95 Products

Product Documents for Human Fas/TNFRSF6/CD95 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fas/TNFRSF6/CD95 Antibody

For research use only

Citations for Human Fas/TNFRSF6/CD95 Antibody

Customer Reviews for Human Fas/TNFRSF6/CD95 Antibody (1)

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  • Human Fas/TNFRSF6/CD95 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    A sandwich assay was developed using the polyclonal antibody AF326 as both capture and detection.
    Human Fas/TNFRSF6/CD95 Antibody AF326

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