Human FCRL2/FcRH2 Antibody

Catalog # Availability Size / Price Qty
AF2048
AF2048-SP
Detection of FCRL2/FcRH2 in Human B Cells by Flow Cytometry.
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Human FCRL2/FcRH2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FCRL2/FcRH2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) IRTA5 is observed and less than 2% cross-reactivity with rhITRA1, rhIRTA2, and rhIRTA3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2
Glu15-Asp395
Accession # Q96LA5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FCRL2/FcRH2 (Catalog # 2048-FC)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of FCRL2/FcRH2 in Human B Cells by Flow Cytometry. View Larger

Detection of FCRL2/FcRH2 in Human B Cells by Flow Cytometry. Human whole blood CD19 + B cells were stained with Goat Anti-Human FCRL2/FcRH2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2048) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Human CD19 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog #
AB‑108‑C).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL2/FcRH2

Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor‑like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B‑CLL) patients (10). FCRL2 up‑regulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).

References
  1. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  2. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
  3. Miller, I. et al. (2002) Blood 99:2662.
  4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  5. Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
  6. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  7. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Huttmann, A. et al. (2006) Leukemia 20:1774.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Li, F.J. et al. (2008) Blood 112:179.
Long Name
Fc Receptor-like 2
Entrez Gene IDs
79368 (Human)
Alternate Names
CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FCRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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