Human FGF-20 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FGF‑20 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) FGF-16 and rhFGF-9 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FGF-20
    Pro3-Thr211
    Accession # Q9NP95
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human FGF‑20 (Catalog # 2547-FG)
  • Neutralization
    Measured by its ability to neutralize FGF‑20-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 10 ng/mL Recombinant Human FGF‑20 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by FGF‑20 and Neutralization by Human FGF‑20 Antibody. Recombinant Human FGF‑20 (Catalog # 2547-FG) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 1-4 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-20

Fibroblast growth factor 20 (FGF-20) is a member of the FGF gene family, which currently contains 22 members. Based on its structure, it is further classified as an FGF-9 subfamily member. All FGF family members are heparin-binding growth factors with a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1). The cDNA of FGF-20 predicts a 211 aa polypeptide without a canonical signal peptide sequence, a feature shared with other members of this subfamily (2‑4). Nevertheless, it is secreted with a molecular weight of 27 kDa (2‑4). FGF-20 is known to bind to heparin (4). No alternate splice forms have been reported. However, three amino acid polymorphisms are known, and single nucleotide polymorphisms in noncoding regions that may effect expression show a strong correlation with a risk of developing Parkinson’s disease (5, 6). Human FGF-20 shows 98% aa identity to bovine FGF-20 and 95% aa identity to both rat and mouse FGF-20. Within the FGF-9 subfamily, FGF-20 is 69% and 63% aa identical to human FGF-9 and FGF-16, respectively. Human FGF-20 is reported to be promiscuous in its selection of receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4 (4, 7, 8). FGF-20 is expressed a variety of cells, including dopaminergic neurons (2), fibroblasts, keratinocytes and breast epithelium (4), and multiple sites in the fetus (2, 7). Finally, the expression of FGF-20 and DKK-1 is regulated by beta -catenin during development and tumorigenesis, implying that FGF-20 may play a role in the oncogenesis induced by the Wnt signaling pathway (9).

  • References:
    1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
    2. Ohmachi, S. et al. (2000) Biochem. Biophys. Res. Commun. 277:355.
    3. Kirikoshi, H. et al. (2000) Biochem. Biophys. Res. Commun. 274:337.
    4. Jeffers, M. et al. (2001) Cancer Res. 61:3131.
    5. van der Walt, J. et al. (2004) Am. J. Hum. Genet. 74:1121.
    6. Genbank Accession # Q9NP95.
    7. Lavine, K.J. et al. (2005) Dev. Cell 8:85.
    8. Ohmachi, S. et al. (2003) J. Neurosci. Res. 72:436.
    9. Chamorro, M.N. et al. (2005) EMBO J. 24:73.
  • Long Name:
    Fibroblast Growth Factor 20
  • Entrez Gene IDs:
    26281 (Human)
  • Alternate Names:
    FGF20; FGF-20; fibroblast growth factor 20
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Fgf20 is required for sensory epithelial specification in the developing cochlea.
    Authors: Hayashi T, Ray CA, Bermingham-McDonogh O
    J. Neurosci., 2008;28(23):5991-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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