Human FGF‑6 Antibody

R&D Systems | Catalog # AB-238-NA

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-6
Gly41-Ile208
Accession # P10767.4

Specificity

Detects human FGF‑6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 17% cross-reactivity with recombinant human (rh) FGF-4 is observed and less then 1% cross-reactivity with rhFGF acidic, rhFGF basic, and rhFGF-5 is observed.  Neutralizes the biological activity of rhFGF-6, but will not neutralize the biological activity of rhFGF acidic, rhFGF basic, rhFGF-4, or rhFGF-5.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human FGF‑6 Antibody

Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody.

Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody.

Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-6 Polyclonal Antibody (Catalog # AB-238-NA). The ND50 is typically 15-25 µg/mL.

Applications for Human FGF‑6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF‑6 (Catalog # 238-F6)

Neutralization

Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 15-25 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References

  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Marics, I. et al. (1989) Oncogene 4:335.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.

    Long Name

    Fibroblast Growth Factor 6

    Alternate Names

    FGF6, HBGF-6, HST2

    Entrez Gene IDs

    2251 (Human); 14177 (Mouse); 170700 (Rat)

    Gene Symbol

    FGF6

    UniProt

    P10767.4

    Additional FGF-6 Products

    Product Documents for Human FGF‑6 Antibody

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Human FGF‑6 Antibody

    For research use only

    Related Research Areas

    FGF Family
    FGF Ligands

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