Human FGFR2 Antibody

Catalog # Availability Size / Price Qty
MAB6843-SP
MAB6843-100
MAB6843-500
FGF R2 in Human Adenocarcinoma.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human FGFR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects all isoforms of human FGF R2 ( alpha and beta, IIIb and IIIc). In direct ELISAs, approximately 25‑50% cross-reactivity with mouse FGF R2 and no cross-reactivity with any isoform of recombinant human (rh) FGF R1, rhFGF R3, or rhFGF R4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 98739
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Kato III human cell line
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize FGF R2 beta -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 beta (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry FGF R2 antibody in Human Adenocarcinoma by Immunohistochemistry (IHC-P). View Larger

FGF R2 in Human Adenocarcinoma. FGF R2 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human FGF R2 Monoclonal Antibody (Catalog # MAB6843) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization FGF R2 beta  Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. View Larger

FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 beta (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB6843). The ND50 is typically 0.1-0.5 µg/mL in the presence of heparin (10 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

Fibroblast Growth Factor Receptor 2 (FGF R2) is one of four closely related transmembrane tyrosine kinases (FGF R1‑4) that function as receptors for the fibroblast growth factor family. Multiple isoforms are generated by alternative mRNA splicing resulting in extracellular domains with three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. In addition, alternative exon usage in the Ig III (membrane proximal) domain results in IIIb or IIIc isoforms.

Long Name
Fibroblast Growth Factor Receptor 2
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
BBDS; BEK; BFR-1; CD332 antigen; CD332; CEK3; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; ECT1; FGF R2; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KGFR; KSAM; K-sam; soluble FGFR4 variant 4; TK14; TK25

Product Datasheets

Citations for Human FGFR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012;30(7):1455-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Keratinocyte growth factor (KGF) enhances postnatal T-cell development via enhancements in proliferation and function of thymic epithelial cells.
    Authors: Rossi SW, Jeker LT, Ueno T, Kuse S, Keller MP, Zuklys S, Gudkov AV, Takahama Y, Krenger W, Blazar BR, Hollander GA
    Blood, 2007;109(9):3803-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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