Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FHL2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FHL2
Lys167-Ile279
Accession # Q14192

Specificity

Detects human FHL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human FHL1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FHL2 Antibody

Detection of Human FHL2 antibody by Western Blot.

Detection of Human FHL2 by Western Blot.

Western blot shows lysates of HT1080 human fibrosarcoma cell line and MG-63 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FHL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FHL2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human FHL2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HT1080 human fibrosarcoma cell line and MG‑63 human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FHL2

FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30‑32 kDa member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40‑92; 101‑153; 162‑212; 221‑275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53‑279. Over aa 167‑279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.

Long Name

Four and a Half LIM Domains Protein 2

Alternate Names

AAG11, DRAL, SLIM3

Entrez Gene IDs

2274 (Human); 14200 (Mouse); 63839 (Rat)

Gene Symbol

FHL2

UniProt

Q14192

Additional FHL2 Products

Product Documents for Human FHL2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FHL2 Antibody

For research use only

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