Human FHL2 Antibody

Catalog # Availability Size / Price Qty
AF4758
AF4758-SP
Detection of Human FHL2 by Western Blot.
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Human FHL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FHL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human FHL1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FHL2
Lys167-Ile279
Accession # Q14192
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human FHL2 by Western Blot. View Larger

Detection of Human FHL2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line and MG‑63 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FHL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FHL2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FHL2

FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30‑32 kDa member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40‑92; 101‑153; 162‑212; 221‑275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53‑279. Over aa 167‑279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.

Long Name
Four and a Half LIM Domains Protein 2
Entrez Gene IDs
2274 (Human); 14200 (Mouse); 63839 (Rat)
Alternate Names
AAG11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; DRALAAG11; FHL2; FHL-2; four and a half LIM domains 2; four and a half LIM domains protein 2; four and a half LIM-domain protein 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM3; SLIM-3; SLIM3aging-associated gene 11

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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