Human Fibulin 5/DANCE Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Fibulin 5/DANCE in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 293904
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fibulin 5/DANCE
    Gln24-Phe448
    Accession # Q9UBX5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human lung
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Fibulin 5/DANCE by Western Blot. Western blot shows lysates of human heart tissue, human lung tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibulin 5/DANCE Monoclonal Antibody (Catalog # MAB3095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Fibulin 5 (Catalog # 3095‑FB) (5 ng/lane) was included. A specific band was detected for Fibulin 5/DANCE at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibulin 5/DANCE
Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2‑4). It is down‑regulated in the adult vasculature but is re‑expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils (6, 9‑11). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 12). Fibulin 5 is down‑regulated in many malignancies (15). When experimentally re‑expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-beta in initiating and promoting epithelial-mesenchymal transition (EMT) (13, 15). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 14).
  • References:
    1. Yanagisawa, H. et al. (2009) J. Cell. Commun. Signal. 3:337.
    2. Nakamura, T. et al. (1999) J. Biol. Chem. 274:22476.
    3. Kowal, R.C. et al. (1999) Circ. Res. 84:1166.
    4. Kuang, P.-P. et al. (2003) Am. J. Physiol. Lung Cell. Mol. Physiol. 285:L1147.
    5. Spencer, J.A. et al. (2005) Proc. Natl. Acad. Sci. 102:2946.
    6. Nakamura, T. et al. (2002) Nature 415:171.
    7. Lomas, A.C. et al. (2007) Biochem. J. 405:417.
    8. Yanagisawa, H. et al. (2002) Nature 415:168.
    9. Wachi, H. et al. (2008) J. Biochem. 143:633.
    10. Hirai, M. et al. (2007) J. Cell Biol. 176:1061.
    11. Hirai, M. et al. (2007) EMBO J. 26:3283.
    12. Sullivan, K.M. et al. (2007) Lab. Invest. 87:818.
    13. Lee, Y.-H. et al. (2008) Carcinogenesis 29:2243.
    14. Loeys, B. et al. (2002) Hum. Mol. Genet. 11:2113.
    15. Schiemann, W.P. et al. (2002) J. Biol. Chem. 277:27367.
  • Entrez Gene IDs:
    10516 (Human)
  • Alternate Names:
    ARMD3fibulin-5; DANCE; DANCEFIBL-5; Developmental arteries and neural crest EGF-like protein; EVEC; FBLN5; Fibulin 5; UP50; UP50FLJ90059; Urine p50 protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Fibulin-5 inhibits hepatocellular carcinoma cell migration and invasion by down-regulating matrix metalloproteinase-7 expression.
    Authors: Tu, Kangshen, Dou, Changwei, Zheng, Xin, Li, Chao, Yang, Wei, Yao, Yingmin, Liu, Qingguan
    BMC Cancer, 2014;14(0):938.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  2. LTBP-2 competes with tropoelastin for binding to fibulin-5 and heparin, and is a negative modulator of elastinogenesis.
    Authors: Sideek M, Menz C, Parsi M, Gibson M
    Matrix Biol, 2014;34(0):114-23.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Functional Assay
  3. Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
    Authors: Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L
    Cancer Res., 2009;69(15):6339-46.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  4. Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation.
    Authors: Lomas AC, Mellody KT, Freeman LJ, Bax DV, Shuttleworth CA, Kielty CM
    Biochem. J., 2007;405(3):417-28.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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