Human Flt-3 Ligand/FLT3L Antibody (40406R)

R&D Systems | Catalog # MAB308R

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 40406R
View all Flt-3 Ligand/FLT3L Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand
Thr27-Pro185
Accession # AAA17999

Specificity

Detects human Flt-3 Ligand in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Flt-3 Ligand/FLT3L Antibody (40406R)

Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody.

Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody.

Recombinant Human Flt-3 Ligand (308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand Monoclonal Antibody (Catalog # MAB308R). The ND50 is typically 0.02-0.06 µg/mL.

Applications for Human Flt-3 Ligand/FLT3L Antibody (40406R)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

UniProt

AAA17999

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Human Flt-3 Ligand/FLT3L Antibody (40406R)

Certificate of Analysis

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Product Specific Notices for Human Flt-3 Ligand/FLT3L Antibody (40406R)

For research use only

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