Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FoxP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP1
Leu231-Gly420
Accession # Q9H334

Specificity

Detects human FoxP1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FoxP1 Antibody

Detection of Human FoxP1 antibody by Western Blot.

Detection of Human FoxP1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human FoxP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxP1 was detected at approximately 82 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FoxP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxP1

Forkhead Box P1 (FOXP1) is a member of the FOX family of transcription factors. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 contains both a DNA binding domain as well as protein-protein interaction domains. FoxP1 can homo or heterodimeize with FoxP2 and FoxP4, with dimerization necessary for DNA binding. FoxP1 shows both oncogenic and tumor suppressive characteristics. Overexpression in lymphomas leads to poor prognosis, whereas, loss of FoxP1 in breast cancer also implicates a poor prognosis.

Long Name

Forkhead Box P1

Alternate Names

QRF1

Entrez Gene IDs

27086 (Human); 108655 (Mouse); 297480 (Rat)

Gene Symbol

FOXP1

UniProt

Q9H334 (Human)

Additional FoxP1 Products

Product Documents for Human FoxP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxP1 Antibody

For research use only

Citations for Human FoxP1 Antibody

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