Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human FoxP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxP1 was detected at approximately 82 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Forkhead Box P1 (FOXP1) is a member of the FOX family of transcription factors. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 contains both a DNA binding domain as well as protein-protein interaction domains. FoxP1 can homo or heterodimeize with FoxP2 and FoxP4, with dimerization necessary for DNA binding. FoxP1 shows both oncogenic and tumor suppressive characteristics. Overexpression in lymphomas leads to poor prognosis, whereas, loss of FoxP1 in breast cancer also implicates a poor prognosis.
Forkhead Box P1
Entrez Gene IDs:
27086 (Human); 108655 (Mouse); 297480 (Rat)
FLJ23741; fork head-related protein like B; forkhead box P1; forkhead box protein P1; FoxP1; glutamine-rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1; QRF112CC4
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