Human FTO Antibody

Catalog # Availability Size / Price Qty
Detection of Human FTO by Western Blot.
1 Image
Product Details
Supplemental Products

Human FTO Antibody Summary

Species Reactivity
Detects human FTO in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 729722
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FTO
Accession # Q9C0B1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.


Recommended Concentration
Western Blot
2 µg/mL
See below
25 µg/mL
Cell lysates spiked with Recombinant Human FTO, see our available Western blot detection antibodies.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FTO antibody by Western Blot. View Larger

Detection of Human FTO by Western Blot. Western blot shows lysates of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FTO Monoclonal Antibody (Catalog # MAB7208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FTO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FTO

FTO (FATSO; also known as Fat Mass and Obesity Associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with Oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.

Long Name
Fat Mass and Obesity Associated
Entrez Gene IDs
79068 (Human); 26383 (Mouse); 291905 (Rat)
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human FTO Antibody

There are currently no reviews for this product. Be the first to review Human FTO Antibody and earn rewards!

Have you used Human FTO Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review