Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Fyn Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241

Specificity

Detects human Fyn in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Fyn Antibody

Fyn antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC).

Fyn in MOLT‑4 Human Cell Line.

Fyn was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Fyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3574) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Fyn Antibody

Application
Recommended Usage

Immunocytochemistry

10-20 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat leukemic T cell line, and Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fyn

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Long Name

FYN oncogene related to SRC, FGR, YES

Alternate Names

SLK, SYN

Entrez Gene IDs

2534 (Human)

Gene Symbol

FYN

UniProt

P06241

Additional Fyn Products

Product Documents for Human Fyn Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fyn Antibody

For research use only

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