Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 222228
View all GATA-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # CAA64997

Specificity

Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) GATA-1 or rhGATA-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GATA‑6 Antibody

GATA-6 antibody in HCT-116 Human Cell Line by Immunocytochemistry (ICC).

GATA‑6 in HCT‑116 Human Cell Line.

GATA-6 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GATA-6 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

GATA‑6 in HT‑29 Human Cell Line.

GATA-6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA‑6 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed KATO‑III human gastric carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and HT-29 human colon adenocarcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human GATA‑6

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-6

GATA-6 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-6 is expressed in visceral and parietal endoderm and other tissues. It interacts with several additional proteins to regulate gene expression in heart, lung, gut and gonads (1).

References

  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Alternate Names

GATA6

Entrez Gene IDs

2627 (Human)

Gene Symbol

GATA6

UniProt

CAA64997

Additional GATA-6 Products

Product Documents for Human GATA‑6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑6 Antibody

For research use only

Citations for Human GATA‑6 Antibody

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Protocols

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